Bibo

LifeStevia Bibo is a high concentration syrup obtained from the leaves of Stevia rebaudiana Bertoni Morita II.

The dry leaves are brewed in water to extract steviol glycosides. The aqueous extract is, then, passed through ion exchange and filtration techniques for purification from other particles. Methods for concentration of steviol glycosides are applied. The final product may be dried up.

Stevioside and rebaudioside A are the component glycosides of principal interest for their sweetening and falvor properties. Associated glycosides also include rebaudioside C, dulcoside A, rubusoside, steviolbioside, and rebaudioside B generally present in preparations of steviol glycosides at lower levels than stevioside or rebaudioside A.

Chemical name Stevioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Rebaudioside A: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-6-en-8-oic acid, β-D-glucopyranosyl ester
CAS. number Stevioside: 57817-89-7
CODEX INS

 

Rebaudioside A: 58543-16-1960
Chemical formula Stevioside: C38H60O18
Rebaudioside A: C44H70O23
Structural formula The seven named steviol glycosides:
Compoundname                        R1                              R2
SteviosideβGlcβGlcβGlc(2→1)
Rebaudioside A                      βGlcβGlcβGlc(2→1)
                                                                                        !
                                                                                βGlc(3→1)
Rebaudioside C                     βGlcβGlcαRha(2→1)
                                                                                         !
                                                                                  βGlc(3→1)
Dulcoside A                            βGlcβGlc-α-Rha(2→1)
Rubusosideβ-Glcβ-Glc
SteviolbiosideH                              β-Glc-β-Glc(2→1)
Rebaudioside B                       H                              β-Glc-β-Glc(2→1)
                                                                                           !
                                                                                    β-Glc(3→1)
Steviol (R1 = R2 = H) is the aglycone of the steviol glycosides.Glc and Rha represent, respectively, glucose and rhamnose sugar moieties.
Formula weight Stevioside: 804.88
Rebaudioside A: 967.03
Assay Not less than 95% of the total of the seven named steviol glycosides on dried basis.
DESCRIPTION Light yellow powder, having a slight characteristic odour.About 200 – 300 times sweeter than sucrose.
FUNCTIONAL USES Sweetener
CHARACTERISTICS
IDENTIFICATION
Solubility (Vol. 4) Freely soluble in water and in ethanol
Stevioside and rebaudioside A The main peak in the chromatogram obtained by following the procedure in Method of Assay corresponds to either stevioside or rebaudioside A.
pH (Vol. 4) Between 4.5 and 7.0 (1 in100 solution)
PURITY
Total ash (Vol. 4) Not more than 1%
Lossondrying (Vol. 4) Not more than 6% (105°, 2h)
Residual solvents (Vol. 4) Not more than 200 mg/kg methanol(Method I in Vol. 4, General Methods, Organic Components, Residual Solvents)
Arsenic (Vol. 4) Not more than 1 mg/kgDetermine by the atomic absorption hydride technique (Use Method II to prepare the test (sample) solution)
Lead (Vol. 4) Not more than 1 mg/kgDetermine using an AAS/ICP-AES technique appropriate to the specified level.The selection of sample size and method of sample preparation may be based on the principles of the methods described in Vol. 4 (under “General Methods, Metallic Impurities).
METHOD OF ASSAY Determine the percentages of the individual steviol glycosides by high pressure liquid chromatography (Volume 4).StandardsStevioside, >99.0% purity and rebaudioside A, >97% purity (available from Wako pure Chemical Industries, Ltd.Japan).

Mobile phase

Mix HPLC-grade acetonitrile and water (80:20). Adjust the pH to 3.3 +/- 0.2 with acetic acid (100% reagent grade). Filter through 0.22 μm Millipore filter or equivalent.

Standard solutions

(a) Accurately weigh 50 mg of dried (105°, 2 h) stevioside standard into a 100-ml volumetric flask. Dissolve with mobile phase and dilute to volume with mobile phase.

(b) Repeat with previously dried rebaudioside A standard.

Sample solution

Accurately weigh 60-120 mg of dried (105°, 2 h) sample into a 100-ml volumetric flask. Dissolve with mobile phase and dilute to volume with the mobile phase.

Chromatography Conditions

Column: Thermo NH2 or equivalent (length: 15-30 cm; inner diameter: 3.9-4.6 mm)

Mobile phase: A 80:20 mixture of acetonitrile and water (see above)

Flow rate: Adjust so that the retention time of rebaudioside A is

about 7 min.

Injection volume: 10-20 μl

Detector: UV at 210 nm

Column temperature:  ambient

Procedure

Equilibrate the instrument by pumping mobile phase through it until a drift-free baseline is obtained. Record the chromatograms of the sample solution and of the standard solutions.

The retention times relative to rebaudioside A (1.00) are:

0.45-0.48 forstevioside                    0.12-0.16 forrubusoside

0.25-0.30 for dulcoside A                 0.35-0.41 for steviolbioside

0.63-0.69 for rebaudioside C            0.73-0.79 for rebaudioside B

Measure the peak areas for the seven steviol glycosides from the sample solution (the minor components might not be detected).

Measure the peak area for stevioside for the standard solution.

Calculate the percentage of each of the seven steviol glycosides, X, in the sample from the formula:

%X = [WS/W] x [fXAX/AS] x 100

where

WS is the amount (mg) of stevioside in the standard solution

W is the amount (mg) of sample in the sample solution

AS is the peak area for stevioside from the standard solution

AX is the peak area of X for the sample solution

fXis the ratio of the formula weight of X to the formula weight of stevioside: 1.00 (stevioside), 0.98 (dulcoside A), 1.20 (rebaudioside A), 1.18 (rebaudioside C), 0.80 (rubusoside), 0.80 (steviolbioside), and 1.00 (rebaudioside B

Calculate the percentage of total steviol glycosides (sum the seven percentages).

 

 

MANUFACTURING Sweet Harvest uses leaves of Stevia Rebaudiana Bertoni produced in a vertically integrated business model with proprietary and controlled farms. Seedlings are cultivated from a mother plantation and nursed in-house to select best individuals guaranteeing the best qualities. Leaves are dried and transported to the manufacturing facilities in bags of25 kilograms. In the manufacturing facility leaves are brewed into water as the only ingredient. No alcohols or chemicals of any kind are added. Water is demineralized and highly purified. Final syrup is pasteurized at packing.
ALLERGENICS Stevia has been approved by FDA and the European Parliament without restriction for allergenic agents as it is also reported in the CODEX Alimentarius.Sweet Harvest used only water for extraction.
GMO No GMO materials are used throughout the process.
NUTRITIONAL FACTS Steviol Glycosides have zero calories as confirmed by Joint FAO/WHO panels.